Genome Research in Next Generation :
High-throughput Genome Analysis Service
The
High-throughput Genome Analysis Core Facility provides high-throughput
sequencing services to researcher in the academic, industrial
communities. Currently, this core has three high-throughput sequencing
platforms: 1) capillary gel electrophoresis system (conventional Sanger
sequencing), 2) Genome sequencer FLX (454 sequencing), 3) Illumina
Genome Analyzer II (Solexa sequencing). Eight service items, namely: 1)
shotgun library construction, 2) PCR product sequencing, 3) large insert
sequencing, 4) whole genome sequencing, 5) EST/plasmid sequencing, 6)
BAC end sequencing, 7) gel electrophoresis, and 8) whole genome
sequencing by GS FLX have been opened to the research community in
Taiwan.
The high-throughput genome sequencers can be used both for de novo
sequencing and re-sequencing of bacteria genome and can be applied in
comparative analysis of bacteria genome, virus sequencing and in
variance strain analysis of microorganisms. We have conducted both the
conventional Sanger sequencing and the 454 sequencing to sequence two
strains of A. baumannii and find a cost-effective combination of these
two technologies to achieve low cost de novo sequencing for genomic
research. In addition to whole genome sequencing, key technologies for
ChIP sequencing, RNA sequencing and metagenomic analysis by 454
sequencing and Solexa sequencing have been also developed. These
high-throughput genome analysis technologies will enable researches to
carry out genome-wide studies on medical sequencing, pathogen
re-sequencing, genome re-sequencing, mapping gene regulatory region,
transcriptome profiling, metagenomic analysis, and small noncoding RNA
identification.
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