Sequencing Core Facility
National Research Program for Genomic Medicine
Introduction
Sequencing capacity
Service item
User priority
Service charge
Service application
Organization
Service information
 

Service Items

  
  The basic genomics core will provide sequencing service of EST and genomic clones.


I. EST Sequencing Service

Depending on the entry materials, the service can be in two categories:
E1 service comes as a cDNA plasmid clone library and requires picking;
E2 service arrives in bacterial clone (96-well) plates. We will serve batch of samples that is at least 400 samples.

Entry E1: Plasmid Library E2: Clone Plates (500-2000 clones)
Phage test 3 days 3 days
Picking 5 days 0 day (500-2,000 clones)
Template preparation 2 days (500-2,000 clones) 2 days (500-2,000 clones)
Reaction 2 days (1,000-4,000 reactions) 2 days (1,000-4,000 reactions)
Sequencer machine time 2 days (1,000-4,000 lanes) 2 days (1,000-4,000 lanes)
Data analysis 1 day 1 day
Total time* 15 working days 10 working days

* When all facility is in full operation on July 1, 2003. The same time frame will be applied before July, 2003, except that maximal output for E1 and E2 will be limited to 1000 clones per day between July 1, 2002 and June 30, 2003.


II. Genomic Sequencing Service

We will be serving the community for sequencing large-insert clones (10 kb or above), or an entire organism (bacteria or fungus).

Entry G1: Large-Insert Clones1 G2: Genome2
Shotgun library 15 days 25 days
Picking 5 days (1,500 clones) 15 days (30,000 clones)
Template preparation 2 days (1,500 clones) 15 days (30,000 clones)
Reaction 2 days (3,000 reactions) 15 days (60,000 reactions)
Sequencer machine time 2 days (3,000 lanes) 15 days (60,000 lanes)
Data analysis 1 day 15 days
Total time 27 working days 100 working days

1. Based on a typical BAC clone insert of 150 kb. Service starts July 1, 2002.
2. Based on a typical bacteria genome size of 3,000 kb. Service starts July 1, 2003.

  
Sponsored by NSC NRPGM
Supported by NSC ¡@NHRI
Operated by YMU YMGC / Sequencing Core Facility
Tel : +886-2-2826-7319
Fax: +886-2-2820-0552
Contact:
Updated: 2008-07-03